FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55448279
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT924070.1666544059915728No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG687550.1239984382563073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA147950.025.34887323
ACGCTAC149950.024.9357624
CGCTACC159100.023.43099625
AAAACGC172100.021.89423221
ACCGCGT208150.021.4839326
AGACCGC211650.021.2806054
GACCGCG213350.020.9632595
GAACGAT216750.020.89599645
GAAAACG195700.020.67931220
CCGCGTT217950.020.4972787
CAGACCG225400.020.2322143
AAACGCT188550.020.02154522
CGCGTTC223400.019.9972328
TCACGCT236550.017.9536538
GTTTTCG226450.017.91438145
TTCACGC245800.017.4335947
AGAACGA262250.017.41397344
ACGCTCA254050.017.36329810
TCAGACC270150.017.2817342
CGTGGTT235450.017.26786441
Produced by FastQC (version 0.11.5)