FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851511

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851511
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55448279
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG856270.15442679474325974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG261750.016.47240645
GGCATAG299200.014.44390435
GGGAGGT441200.014.1777863
ATAGGGG316450.013.8061338
GGAGGTC435250.013.7511824
GAGCTTA319250.013.69446144
GTCACTC432300.013.5401668
GGTCACT437150.013.3683027
TGGCATA331900.013.09558634
ACTCTCC455250.012.85762711
TAGCGTA12000.012.56043237
CATAGGG349300.012.48792437
AGGTCAC496000.012.11292656
GAGGTCA500200.012.1057765
GCATAGG364550.011.97788736
TCAGACG100250.011.8055442
TGGGAGG392550.011.7501532
ACCCGGC91250.011.14623455
CACTCTC530450.011.0730310
TCTCCCC540350.010.8117913
Produced by FastQC (version 0.11.5)