FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49166106
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG818480.1664724068243273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG246800.016.80064445
GTCACTC350600.016.3104448
GGTCACT352550.016.2970647
GGGAGGT378600.016.1021523
ATAGGGG262450.016.04326638
GGCATAG259000.016.03341335
AGGTCAC370100.015.8284956
GGAGGTC377100.015.8077084
GAGGTCA391500.015.3222325
ACTCTCC382800.015.08426511
GAGCTTA280000.014.96500644
TGGCATA289200.014.49947534
CATAGGG297900.014.12678937
TCACTCT426000.013.6133929
CACTCTC429450.013.37291510
TGGGAGG334550.013.2377322
GCATAGG319050.013.14119336
GTTGTCG30800.012.85800426
TCTCCCC473900.012.37316313
TAGGGGA469900.012.258409539
Produced by FastQC (version 0.11.5)