FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51362965
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1527280.29735043527958327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT257500.028.94162245
AGACCGC278650.026.9039424
CAGACCG291800.025.8225943
AGAACGA290900.025.7500444
GACCGCG294550.025.4356295
ACCGCGT303300.024.660326
CCGCGTT312700.023.9837597
CGCGTTC315900.023.7620548
TACGGAG314500.023.61921334
AATACGG315550.023.5838832
ATACGGA318650.023.38270233
TCAGACC340300.022.4464552
GCGTTCT341350.022.0759459
ACGGAGA342850.021.6395435
CAATACG350400.021.28324931
AAGAACG353800.021.1655543
CTCAGAC397900.021.1388721
CGGAGAG368100.020.15666636
CCCAATA376450.020.08531629
CCAATAC397050.019.19651230
Produced by FastQC (version 0.11.5)