FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47436980
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC75650.011.0684745
GGCATAG111350.010.84578835
AGCTTAG116450.010.48758445
ATAGGGG117250.010.43437238
TCACCCG94400.09.918853
ACGGACA83250.09.80837618
CATAGGG124300.09.73400137
GCATAGG130350.09.213160536
GGGAGGT219200.09.2004163
ATAGCTC82900.09.16945239
TAGCGTA8950.09.04606537
CGGACAG90450.08.9773619
TCAGACG75150.08.9543592
CACCCGG100250.08.8686174
ACTCTCC197900.08.86766611
GTCACTC196850.08.765688
GAGCTTA140150.08.74588744
GGAGGTC209450.08.7153264
TGGCATA142900.08.67154334
CGGACAC98650.08.43855213
Produced by FastQC (version 0.11.5)