FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47867387
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC490550.10248104831792886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC85650.023.4774534
ACCGCGT91150.022.0608226
GACCGCG94000.021.3680275
CAGACCG99050.020.7781773
TTCGACG17900.018.9742576
TGCGCGT65050.018.73229245
GTCGCTA63600.018.22055227
TTCGGCG135800.018.18268844
CGACGTC65400.017.8536923
CGCTATG65100.017.83715229
TCGGCGC140100.017.66853345
TCGCTAT66650.017.62348228
ATGCGCG69750.017.52293644
TCAGACC134450.016.0771262
TCGACGA22850.016.0450947
ACGTCGC73650.016.00723825
CCGTACG158750.015.8263324
GCGACGT74050.015.7977222
CCGCGTT147350.015.731111
CGTACGC159700.015.7181025
Produced by FastQC (version 0.11.5)