FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52366446
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3159620.6033672783522487No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA937300.17898866002859923No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG931100.17780469577790328No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC529920.10119457027883848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT580100.027.05360645
AGAACGA711400.022.16166544
GACCGCG710350.022.136875
ACCGCGT715700.022.0123546
AGACCGC710700.021.9808034
CAGACCG717050.021.8300533
CCGCGTT734200.021.473027
CGCGTTC744250.021.186088
TCAGACC759950.020.9974212
CTCAGAC830300.020.0262451
GCGTTCT790350.019.9987149
AAGAACG816150.019.25939243
AATACGG812500.019.16376132
ATACGGA814000.019.1345433
TACGGAG806300.019.12192234
CAATACG832300.018.85175931
TTCGACG25800.018.7470176
ACGGAGA838750.018.3741135
CGTTCTC879050.018.33648310
CCCAATA870450.018.25819829
Produced by FastQC (version 0.11.5)