FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851523

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851523
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52366446
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG763880.14587203416477795No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA734740.140307402186507No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN648020.12374717963483715No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG529460.10110672776991586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG190450.014.85292345
ATAGGGG205150.013.83088738
GTTGTCG35450.013.64768626
GGCATAG210650.013.25677935
GGGAGGT314550.012.6758693
GAGCTTA229700.012.461344
GGTCACT300300.012.35104757
GTCACTC302750.012.2662868
GGAGGTC322350.012.1255174
AGGTCAC324300.011.72804456
TGGCATA243150.011.71678134
CATAGGG241300.011.6938537
ACTCTCC319200.011.67712511
GAGGTCA342650.011.4677925
GCATAGG253400.011.1533836
ATATAGC50600.010.9348676
AGGGATC185350.010.7981932
TGTCGTT46400.010.47577928
TAGGGGA371750.010.1510839
TCACTCT377000.010.125129
Produced by FastQC (version 0.11.5)