FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48311337
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA695140.14388755169412928No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG527950.10928076778334658No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG144000.015.85761545
ATAGGGG150350.015.17320738
GGCATAG151750.014.97378335
GTCACTC236150.013.5491658
CATAGGG169100.013.4242237
GGTCACT232150.013.395567
GAGCTTA174800.013.20508444
TGGCATA175800.012.92521334
ACTCTCC247700.012.79846811
GCATAGG183300.012.39647536
GGGAGGT278700.012.1483743
AGGTCAC282200.011.8093366
GAGGTCA286050.011.6216795
GGAGGTC286200.011.4600714
GTTGTCG26700.010.95380726
AGGGATC150450.010.8786422
TCACTCT304800.010.7038829
TAGGGGA294200.010.53014139
CACTCTC301900.010.50853310
TAGGCTA67800.010.28769310
Produced by FastQC (version 0.11.5)