FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46307187
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACGGTGAGCTTGCTATAGAGGAAGAAGGAGCCGTCGGAGTCCAGCACGG525050.11338412760852869No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG511590.11047745137272105No Hit
CTGGGCTTGTGATTCACGTTGCAGATGTAGGTCTGGGTGCCCAAGCTGCTG510110.11015784655630237No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCC478450.10332089487534624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT86750.022.583186
AGACCGC89450.022.0021024
CCGCGTT95550.020.6210087
AACGCTA161650.020.52356323
CAGACCG98150.020.4414163
ACGCTAC164400.020.11197724
CGCGTTC97800.019.985618
CGCTACC166000.019.8232825
AAAACGC177200.018.87485921
GACCGCG110350.017.7738115
GAAAACG189600.017.64042720
AAACGCT193600.017.2991922
CGTGGTT189500.017.20423741
GTTTTCG194650.016.7146845
TCACGCT205850.016.684948
TTCACGC212700.016.3908187
TCGTGGT201100.016.26778840
TCAGACC129300.015.9865012
GCCGGCT217150.015.9614591
CACGCTC214400.015.9356739
Produced by FastQC (version 0.11.5)