FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46307187
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTATCCCAGCGACATCGCCGTGGAGTGGGAGAGCAATGGGCAGCCGGA466890.10082452212007609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG151150.014.70584445
GGGAGGT246000.014.2149793
GGCATAG155550.014.16151435
GTTGTCG23100.013.73140726
GGAGGTC239750.013.6835934
ATAGGGG163900.013.62167738
TGGCATA169950.013.11938234
GAGCTTA175150.012.82017444
AGCGCTC66000.012.0005959
GTCAGGA151300.011.6631611
CATAGGG197100.011.395720537
GCATAGG202850.011.028316536
CGTCAGT129550.010.7371811
TGGGAGG227450.010.5958822
ACGCGCG17250.010.56333420
TGTCGTT31600.010.53630128
GCGTCGA19350.010.34938411
TAGGGGA308650.010.244904539
ACTCTCC325500.010.00974611
GGGGAGC321450.09.98398741
Produced by FastQC (version 0.11.5)