FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57451285
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA888460.15464580122098226No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG701690.12213651966183177No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG653920.113821649071905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG247400.015.07681745
ACCCGGC113450.014.5026825
GGCATAG257600.014.20914735
TCACCCG123150.013.7091193
GGGAGGT418550.013.45082953
GGAGGTC380900.013.4323354
TGGCATA280650.013.1864334
GAGCTTA288350.013.06050544
ATAGGGG292000.012.75865738
AGCGCTC77750.012.6188619
ACGGACA129950.012.53477718
TCAGACG131300.012.1903012
AGGGATC204150.012.1187512
CGGACAC132800.012.08075713
CCGGACA136750.012.06121512
ACTCTCC416850.011.96247411
CATAGGG310650.011.91306637
CACCCGG150100.011.5321074
GCATAGG324150.011.43072936
ATAGCTC133650.011.24442539
Produced by FastQC (version 0.11.5)