FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851531

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851531
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54690104
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG548470.10028688188268942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG180350.014.85920945
ATAGGGG187000.014.73932138
GGCATAG183300.014.71975435
GGGAGGT314450.013.5827983
GGTCACT306650.013.2175817
GGAGGTC316400.013.2004454
GTCACTC310400.013.152238
TGGCATA211650.013.02500834
CATAGGG214350.012.70171637
GAGCTTA218650.012.43115744
ACTCTCC329400.012.38760211
GAGGTCA349450.012.1839535
GCATAGG229250.011.92640736
AGGTCAC357450.011.8298036
GCGTAGG110400.011.47577436
CGTAGGG111200.011.41234337
GGCGTAG111350.011.37789835
GTTGTCG26250.011.3157526
TAGGGGA366050.011.05187239
CACTCTC378400.010.80705110
Produced by FastQC (version 0.11.5)