FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56768950
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1068800.18827193386525556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG40200.024.6157916
AACGCTA315900.021.76371623
ACGCTAC317850.021.58066724
CCGGCTT340750.021.5184212
TCACGCT333800.021.0952158
CGCTACC327400.020.90309125
GCCGGCT314550.020.7071931
TTCACGC343350.020.4560687
AAAACGC337950.020.3703321
CGGTATA64650.020.07217820
TCGACGA51350.020.0591377
GTTCGAC48950.019.8879555
AAACGCT354150.019.5274322
GAAAACG354700.019.41472220
CGACGAG51000.018.7856678
CGGCTTC411450.018.355083
ACGCTCA387750.018.26451310
AGACCGC119550.017.9461064
CACGCTC404400.017.4569119
GACCGCG124600.017.0369535
Produced by FastQC (version 0.11.5)