FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56768950
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG202550.014.05536445
GTTGTCG37850.013.79782926
ATAGGGG218950.013.13355738
GGGAGGT300550.013.0266863
GGCATAG225300.012.49514235
GGAGGTC321750.011.950584
GAGCTTA239950.011.88589744
GTCACTC313750.011.8149898
ATATAGC51000.011.51197056
GAGGTCA347600.011.0987495
TGTCGTT45900.011.03448328
ACTCTCC338350.010.98710211
TGGCATA260900.010.94529934
AGGTCAC350700.010.9298246
GGTCACT346000.010.7337227
CATAGGG269300.010.5281537
GCATAGG278300.010.195836
TCACTCT374350.010.1354529
CACCGAA309000.09.993237545
TCAGACG105300.09.9364262
Produced by FastQC (version 0.11.5)