FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851536

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851536
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46345447
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT667470.14402061976012445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT130650.025.84195745
AGACCGC132550.025.5787664
CAGACCG140000.024.3622443
ACCGCGT144050.023.59926
CCGCGTT149900.022.633197
AGAACGA152000.022.2065944
CGCGTTC153800.022.1616638
AATACGG155100.021.65950232
ATACGGA156300.021.50509833
TACGGAG159750.021.09700434
GACCGCG167350.020.2731725
TCAGACC176100.019.8667562
GCGTTCT173100.019.7297139
CAATACG176250.019.14980931
ACGGAGA181850.018.62011735
AAGAACG182950.018.46213743
CCCAATA189700.018.06510529
TTCGGCG68000.017.47266644
CTCAGAC236350.017.0979161
CGGAGAG200700.016.8712936
Produced by FastQC (version 0.11.5)