FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46345447
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG516430.1114305791462104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG155000.017.67566945
GGCATAG162450.016.72991635
ATAGGGG179850.015.40898838
GAGCTTA180350.015.40320444
CATAGGG184500.014.71590837
TGGCATA188200.014.49031434
AGCGCTC86000.014.3908429
GGGAGGT313400.014.1941153
GGAGGTC307350.013.6442824
GCATAGG201450.013.51334136
TAGGGGA301900.013.35203139
ACCCGGC83300.013.159595
AGCATAG103000.012.68817445
CGTAGGG103450.012.67693437
GCGTAGG103700.012.5832836
GTCAGGA166400.012.518771
GGCGTAG105600.012.42084535
ACTCTCC334950.012.30609111
ACGGACA88850.011.95053918
TCACCCG93800.011.9041713
Produced by FastQC (version 0.11.5)