FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47388800
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2581840.5448207171314741No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC2064380.43562613950975754No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT2026590.42765168140995347No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG1274060.2688525558781822No Hit
CGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTC918720.1938685934229185No Hit
GTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGC905770.19113588020798164No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCT865340.1826043284489162No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT836020.17641721250590858No Hit
GTGGGTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTC810560.17104463501924505No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCC794200.16759234249442906No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT779090.16440382537646026No Hit
CGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGT762830.16097263488419206No Hit
GCAGGTTTGGTAGTTTAGGACCTGTGGGTTTGTTAGGTACTGTTTGCATTA602940.1272325950435546No Hit
CCCCAACCGAAATTTTTAATGCAGGTTTGGTAGTTTAGGACCTGTGGGTTT601200.1268654196772233No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGT584630.12336881288405699No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG567410.11973504287933015No Hit
GCGGGTCTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATT537580.11344030657032884No Hit
CGGCGCTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTT524650.11071181376190153No Hit
GTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTC505040.10657370517928287No Hit
GTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTGGTT488990.10318682895536498No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG477880.1008423931393072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT568250.025.9221781
TCGGCGC411150.025.06215145
TTCGGCG418400.024.7784444
TCACGCT649150.024.5724228
AACGCTA653400.024.0226723
ACGCTAC653850.023.98903324
GTTAGTT402600.023.9402644
GTTTTCG652450.023.87607845
CGCTACC662600.023.61461425
CGTGGTT670000.023.41202241
AAAACGC670950.023.40693321
GAAAACG677000.023.16437720
CACGCTC687450.023.1379599
TTCACGC692450.023.087857
AAACGCT683600.022.98368822
TCGTGGT688000.022.8354740
CCGGCTT697900.022.8167882
CGGCTTC731300.022.482373
ACGCTCA754700.021.48160210
AGAAAAC738500.021.3720619
Produced by FastQC (version 0.11.5)