FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47388800
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG1549400.32695489229522584No Hit
CGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACT1347540.28435832939428723No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC1308180.276052569383483No Hit
CGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT1236870.26100470997366465No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC1139110.24037536295495981No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA874800.18460058072793573No Hit
ATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAA865360.18260854885542574No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG861780.1818530960902154No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG851450.17967325612803026No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA833950.17598040043216961No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCT811170.17117335741778647No Hit
CAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGA809620.17084627591329596No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGA682900.14410578026875548No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCAC591640.12484806536565603No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCG589330.12436060841380242No Hit
GTCCAAGTGGCATAGGGGAGCTTAGGGCTCTGCCCCATGATGTACAGTCCC577490.12186212776014585No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT567090.11966751637517725No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA532950.11246328246336686No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTT532910.11245484165034778No Hit
GACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAA503440.10623607265851846No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT499870.10548273009656291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGACG293050.016.1042442
GGGAGGT361600.014.9553333
GTCACTC347600.014.82548
GGTCACT349400.014.7893717
TGCCCGT499300.014.66976944
AGGTCAC383950.013.8126386
TAGCGTA12900.013.77949337
ACTCTCC374100.013.74457411
ATAGCTC80550.013.60306439
TGGGAGG324700.013.5987912
AGGGATC198200.013.4327422
GAGGTCA401950.013.3591925
GCCCGTG560950.013.13998845
GGAGGTC409400.013.1021284
TCACTCT398300.013.0702999
ATCAGAC367950.012.9054081
TAGCTCT86800.012.75305240
CACTCTC413900.012.53497310
CGTCGAT69350.012.4495512
TGCGTCG70100.012.34598410
Produced by FastQC (version 0.11.5)