FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76725545
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18718312.439645101250177No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1602250.20882875449108898No Hit
CTGGCCTACTGGGCTGACATTAACTACAATTATGGGAAATGCAAAAGTTGT862670.11243582564320657No Hit
GTTTAAACAGTTCAGTGATCTTTAGTGCATTGTTTATGTGTGGGTTTCTCT858120.11184280281098036No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTC7400.027.62674910
ATACGCG4000.027.5355831
CGCATAG27500.024.30147240
TCGCGTA803.0043648E-622.47802431
TACGCTA27500.020.9235123
CGCGTAA2200.020.433724
AATACGC29450.020.2965621
TCGCGCA9250.020.158088
TCGCGAG3650.019.0792583
CGCGCAT9900.019.061549
ACGCATA19900.018.55592539
AATCGCG4900.018.3387436
CGTATCG18950.018.25709310
GCGCATA16100.017.87005839
TAGCGCG1701.8608262E-917.18834924
TTCGCGC5100.016.74852845
TACGCGC6600.016.6882332
TTCGCGA2750.016.3379612
CGTTCGC9400.016.260731
ATACGCT33250.016.15531222
Produced by FastQC (version 0.11.5)