FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52533361
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT831630.1583051196743342No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG634460.12077277903464048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT164850.025.127956
GACCGCG165700.024.9448495
AGACCGC171750.024.2364644
CCGCGTT176200.023.4709457
CGCGTTC181750.022.8647968
CAGACCG180200.022.8628253
TCAGACC218700.019.4888692
GAACGAT229400.018.05667345
TTCGGCG75200.017.56295644
AATACGG234000.017.55734832
GCGTTCT239300.017.4975199
ATACGGA238250.017.25353233
TCGGCGC77450.016.9074845
TACGGAG243100.016.8352734
CAATACG252000.016.42831231
CTCAGAC299900.016.1856141
AGAACGA257850.016.1080144
CGTACGC87000.015.5693865
CCGTACG87250.015.5247764
AAGAACG271300.015.33425843
Produced by FastQC (version 0.11.5)