FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851545

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851545
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52533361
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG921970.1755018111253152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT389000.016.968993
AGCTTAG270150.016.95337745
GGCATAG274300.016.74763535
ATAGGGG278450.016.6837138
GGAGGTC400050.016.3700354
GTTGTCG34500.015.84617126
GTCACTC401950.015.8050548
GGTCACT409700.015.4841697
TGGCATA303750.015.24963634
ACTCTCC424400.015.13351611
GAGCTTA309900.014.88121844
GAGGTCA461750.014.3810495
CATAGGG323650.014.26333637
AGGTCAC464200.014.1072136
TGGGAGG354200.013.862732
CACTCTC481600.013.37808810
GCATAGG348050.013.26960736
TCACTCT495500.013.1163429
TAGGGGA488700.012.8524639
CTGGGAG431200.012.8491651
Produced by FastQC (version 0.11.5)