FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50607556
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1961100.3875113036480165No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG885140.17490273586813795No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT592150.11700821908886491No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA544010.10749580556705801No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG507970.10037433935754575No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT393850.025.46198345
ACCGCGT437550.023.0067466
AGACCGC440300.022.9397034
CAGACCG445700.022.768553
GACCGCG443750.022.7055825
CCGCGTT447900.022.4793117
AACGCTA225050.022.47585323
ACGCTAC228350.022.12172324
CGCGTTC456150.022.067928
AGAACGA469650.021.38540344
TCAGACC483450.021.2388212
CGCTACC239350.021.04896425
AAAACGC245850.020.67507421
TCACGCT284000.020.4634678
GAAAACG281200.020.3554620
GCGTTCT504550.019.9725979
AAACGCT265450.019.14839622
AATACGG523450.019.14178332
CTCAGAC595400.018.958231
ATACGGA531050.018.87556833
Produced by FastQC (version 0.11.5)