FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50367314
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3994290.7930321636766257No Hit
CACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCA1180880.23445363792875673No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG624610.12401098061333984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGACT318450.019.691792
AGACTCA341550.018.6617224
CACAGAC367350.017.801861
GTTGTCG30300.017.07770726
CAGACTC377500.016.8791283
GACTCAG384200.016.6307755
AGCTTAG183000.016.13066745
ACTCAGA399750.015.8624396
GGCATAG194350.015.17701735
CCGACAA391350.014.82302840
ATAGGGG204250.014.65842638
GCCGACA397600.014.58485839
GGTCACT271750.014.3828847
AACCCAC412950.014.28163316
GTCACTC283250.014.0364448
AGAACCC424250.014.00177114
GAGCTTA218400.013.58853544
TGCCGAC430500.013.58014738
ACCCACC452600.013.42401117
CGACAAG436100.013.40100741
Produced by FastQC (version 0.11.5)