FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50534971
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG809680.16022172051904413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA212600.020.54158623
ACGCTAC214650.020.3244424
TTCGACG22800.019.9337126
AGACCGC92500.019.7511124
CGCTACC220650.019.64940825
TCACGCT239750.018.9849268
AAAACGC232600.018.92042221
CCGGCTT259550.018.871992
GCCGGCT241500.018.6587051
ACCGCGT98300.018.539726
CAGACCG101750.018.3535863
TTCACGC251350.018.2251267
GACCGCG100900.018.1066025
GAAAACG246400.017.961220
AAACGCT250100.017.60551822
ACGCTCA266400.017.35598610
CCGCGTT108800.016.812547
CACGCTC280600.016.3493799
CGCGTTC111700.016.3156198
CGGCTTC315150.016.227793
Produced by FastQC (version 0.11.5)