FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50534971
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG574000.11358470948761402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG30150.014.77427926
AGCTTAG200900.014.56886845
GGGAGGT296000.014.5344683
ATAGGGG203350.014.52658738
GGCATAG201500.014.50344335
GGAGGTC301400.013.8403724
GAGCTTA222400.013.13016144
TGGCATA229150.012.92013534
GTCACTC323650.012.6807938
ACTCTCC332050.012.50826511
GGTCACT333550.012.1499497
CATAGGG248300.011.86960737
GCATAGG262650.011.28097636
TGGGAGG293250.010.9019062
TGTCGTT43400.010.83397928
ATATAGC51150.010.77836
CACTCTC406750.010.3608510
TAGGGGA389700.010.33392239
TCACTCT418400.010.3036319
GCTAAGT45550.010.22466514
Produced by FastQC (version 0.11.5)