FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44694111
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1082960.24230485309350933No Hit
CCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAA603600.13505134938247232No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG458700.10263097077823072No Hit
CTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGCAG448600.10037116523024701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC213000.024.6260324
CAGACCG219600.024.0293663
ACCGCGT235750.022.2525046
TCAGACC249950.021.4361022
GACCGCG254900.020.622195
TCGGCGC125150.018.51447145
ACGTTCG20100.017.9071316
CTCAGAC340000.017.780421
TTCGGCG131700.017.71317944
AACGCTA140250.017.62944623
ACGCTAC141450.017.48084324
CCGCGTT314850.016.6904037
CGCTACC152550.016.23905425
CTACACG37700.016.1708545
CGCGTTC333900.015.7314278
AAAACGC159600.015.64626121
GAAAACG160250.015.55404220
GTTTTCG165500.015.25105245
TTCGCGC33900.014.8643537
CGTGGTT173500.014.61281141
Produced by FastQC (version 0.11.5)