FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851557

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851557
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54965723
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4164640.757679472350432No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG638720.11620332911840348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG43000.018.10389326
AGCTTAG191850.016.5398245
GGCATAG207550.015.20984535
ATAGGGG213650.015.10054738
TGTCGTT54750.014.38330328
GAGCTTA229250.013.94916744
GGGAGGT335700.013.6760633
ATATAGC63650.013.5044316
GTCACTC316900.013.3615168
CATAGGG240350.013.24627637
TGGCATA244100.013.00632734
GGTCACT327050.012.9889757
GCTAAGT60750.012.88782914
GGAGGTC341800.012.8724424
GTCGTTC61500.012.76810929
GCATAGG249900.012.75797836
ACTCTCC344100.012.59209811
TCGTTCC62950.012.18805930
AGGTCAC361550.012.1547276
GAGGTCA369950.012.1420115
Produced by FastQC (version 0.11.5)