FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48885709
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT495810.1014222786458922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC125400.020.8492324
GACCGCG126500.020.5256425
ACCGCGT128250.020.2280226
CAGACCG131950.020.1382623
CCGCGTT136050.019.0352347
CGCGTTC146850.017.681128
GAACGAT139650.017.33631745
AACGCTA105200.015.91237923
TCGGCGC88000.015.90351845
AATACGG154350.015.68496632
ACGCTAC107250.015.65018424
TTCGGCG88400.015.627933544
AGAACGA158800.015.35904644
ATACGGA158000.015.336925533
TCAGACC181200.015.0869922
ACGTTCG21300.014.8943296
GCGTTCT178050.014.7218329
TACGGAG164150.014.63905834
CGCTACC114200.014.59923325
ATGCGCG33450.014.19292244
Produced by FastQC (version 0.11.5)