FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59132525
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1303540.22044382511993188No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1070630.1810560262731889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG36050.025.9615466
AGACCGC310900.021.023734
TCGACGA45950.020.8577087
GAACGAT313300.020.54143345
GACCGCG317800.020.517315
ACCGCGT319550.020.4526626
CAGACCG331550.019.8365923
AACGCTA315900.019.64254623
ACGCTAC316700.019.58582124
CCGCGTT335150.019.5006647
CGGTATA57500.019.48654420
CGCGTTC338650.019.2924778
TCACGCT333500.019.07778
CGCTACC330050.018.76115825
CGACGAG50300.018.7408188
GCCGGCT306750.018.580581
AAAACGC340950.018.25302321
TTCACGC354200.018.2231927
CCGGCTT354750.018.1143652
GAAAACG350700.017.76480920
Produced by FastQC (version 0.11.5)