FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53117671
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT905390.1704498678038802No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG575790.10839895446470159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT224000.019.29971145
AGACCGC228350.019.00254
ACCGCGT236700.018.360636
CAGACCG241550.018.1223833
GACCGCG241700.017.9994725
CCGCGTT253350.017.1538527
CGCGTTC268850.016.1397828
AGAACGA272100.015.87264644
ACGTTCG21850.015.7513846
TGCGCGT36650.015.69043145
TTCGGCG76800.015.5821444
ATGCGCG37650.015.53406244
TTCGACG18750.015.3563296
TCAGACC292650.015.1963382
TCACGCT219350.015.0749248
AACGCTA214350.014.81788623
ACGCTAC216050.014.75334924
GTCCGTT42350.014.6116141
TACGGAG294300.014.5914834
AATACGG295700.014.54496932
Produced by FastQC (version 0.11.5)