FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53117671
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4049270.7623206973814797No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG556200.10471091625986388No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG180200.015.65869645
GGCATAG192450.014.60122735
GAGCTTA208350.013.47813644
ATAGGGG212950.013.25784738
TGGCATA216200.013.17279834
ACCCGGC108050.013.0667885
GGTCACT327150.012.755867
GTCACTC336200.012.6399578
TCACCCG119100.012.6269743
GGGAGGT376500.012.4988363
ACTCTCC341550.012.48148211
CATAGGG228800.012.37877137
ACGGACA119550.012.08787618
GGAGGTC364550.011.9190024
CGGACAC124600.011.89846313
GCATAGG238650.011.86832436
AGGTCAC369150.011.8068666
ATAGCTC119350.011.62163939
TAGCGTA12600.011.52277637
CCGGACA130550.011.42624612
Produced by FastQC (version 0.11.5)