FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851568

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851568
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52149244
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG970450.18609090478857182No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG29850.025.8484256
AGACCGC124500.022.390884
GACCGCG133050.020.781415
TCGACGA38950.020.3866627
CAGACCG139700.020.2928663
ACCGCGT142000.019.5800786
CGGTATA54150.018.40328620
CGACGAG42250.018.0489468
GCCGGCT321950.016.296411
TCACGCT343150.016.0540148
AACGCTA337350.015.91899123
CTACACG105700.015.87601445
ACGCTAC338050.015.86689924
CCGCGTT178150.015.5940897
TCAGACC194400.015.4509162
CGCTACC346450.015.45074425
GTTCGAC50900.015.2047275
CCGGCTT376350.015.1908462
TTCACGC373150.014.9924037
ACGCTCA371100.014.95393310
Produced by FastQC (version 0.11.5)