FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52392833
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT932850.17804916180043176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG190700.024.1482265
AGACCGC192650.024.020574
ACCGCGT191900.023.9972216
GAACGAT192600.023.65836745
CCGCGTT200750.022.9617257
CAGACCG205600.022.715633
CGCGTTC205200.022.4637728
ATACGGA220000.020.63959333
AATACGG220700.020.60491232
AGAACGA222550.020.5395244
TACGGAG224900.020.05934534
TCAGACC248400.019.2277152
GCGTTCT250200.018.5583959
CAATACG250250.018.24403831
ACGGAGA253600.017.91343735
TTCGACG18250.017.8740626
AAGAACG259450.017.74842843
CTCAGAC316700.017.2554871
CGGAGAG267600.016.84185836
CCCAATA275000.016.78043729
Produced by FastQC (version 0.11.5)