FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52392833
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG142700.014.16969745
ACCCGGC98850.013.9593265
GGCATAG144750.013.87991135
GTCACTC227950.012.9812048
GGTCACT230250.012.8712397
TCACCCG111900.012.65578653
ATAGGGG162650.012.65428138
CATAGGG161050.012.62685437
GAGCTTA168800.012.16541544
AGGTCAC254600.012.1532676
ACGGACA112600.011.94581618
ACTCTCC257000.011.86356211
GCATAGG171950.011.78825336
GGGAGGT303750.011.5261073
CGGACAC118750.011.38634313
ATAGCTC113150.011.371140539
GAGGTCA279550.011.2571465
TGGCATA183100.011.15775934
CACCCGG128750.011.067874
GGAGGTC285000.010.8845874
Produced by FastQC (version 0.11.5)