FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48008327
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG152450.015.83178545
GGCATAG156050.015.49857935
GTCACTC238300.015.0429528
ATAGGGG164600.015.01886838
GGTCACT239850.014.7958097
GAGCTTA175350.013.90532544
AGGTCAC264950.013.7174246
ACTCTCC266150.013.61898511
CATAGGG179750.013.49029537
GAGGTCA284450.013.3735475
GGGAGGT306650.013.0163993
GGAGGTC292800.012.9002874
GCATAGG188100.012.89355436
TGGCATA196250.012.53157434
GCGTAGG91850.012.19811836
CGTAGGG92450.012.01964637
GGCGTAG93650.011.98781335
TAGGGGA302850.011.77252739
TCACTCT316200.011.57836159
ACCCGGC80950.011.5119615
Produced by FastQC (version 0.11.5)