FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47525220
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT36750.017.7712881
TTCGACG16200.016.9442776
GCACTAC63250.016.50552425
CCCGTAT32100.016.0660341
AACGCTA93150.015.6517923
ACGCTAC95550.015.32929424
TCACGCT116300.014.8770918
ACGTTCG18650.014.8389956
TTCGGCG64600.014.69776544
TCGGCGC62850.014.60583145
CGCTACC101050.014.5172125
CTCCGTT70900.014.261931
CTCGCGT8300.014.1092361
CTCGGTT82800.013.844131
ATGCGCG23950.013.80966744
TGCGCGT24600.013.62769945
GTTCCTA78250.013.37048915
GAAAACG106700.013.26349820
TACGGTA78900.013.26011744
ACGCTCA138150.013.24089810
Produced by FastQC (version 0.11.5)