FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851575

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851575
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47525220
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG501910.10560919023625771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG145450.016.6718345
GGCATAG164200.014.67226835
ATAGGGG176100.014.11503738
GAGCTTA175400.013.94049444
CATAGGG184650.013.19344637
TGGCATA188400.012.91904634
GCATAGG193050.012.60771936
GGGAGGT290200.012.3536653
ACCCGGC87900.012.1361285
GGAGGTC275300.012.0576794
TCACCCG91850.011.93238453
ACGGACA91850.011.56377418
ACTCTCC287550.011.49753211
CGGACAC91800.011.2527913
TAGGGGA302350.010.68372339
GGTCACT308400.010.4501627
GTCACTC318400.010.3340388
CGGACAG102600.010.13250419
ACACGGA103400.09.989816
CACGGAC106250.09.97584717
Produced by FastQC (version 0.11.5)