FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51367091
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC90100.010.7869815
ACGGACA98650.010.28689718
ATAGCTC90100.010.0381439
GTTGTCG31900.09.94554726
CGGACAC98550.09.63683513
CGGACAG102050.09.56888519
TCAGACG101850.09.5445422
AGCTTAG104600.09.33595545
TGTCGTT35500.09.253928
CCGGACA106500.09.12869212
TAGCTCT102300.09.10502940
ACACGGA105650.09.09618816
GGCATAG104200.09.06810635
ATAGGGG111900.08.86668638
AGGGATC153300.08.8513112
CGTCGAT26300.08.64251212
GACACGG112300.08.61740815
CACCCGG117350.08.589114
TCACCCG119750.08.5689763
CACGGAC117350.08.5337417
Produced by FastQC (version 0.11.5)