FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48266723
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1776040.36796365893744226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT307000.027.8295445
ACCGCGT359150.023.7775146
AGAACGA362900.023.58123244
AGACCGC363350.023.5707574
GACCGCG365000.023.4395585
CCGCGTT370950.023.0150837
CAGACCG375450.022.9129543
CGCGTTC375250.022.745368
AATACGG398450.021.4619632
TCAGACC407350.021.361552
ATACGGA400000.021.3125233
TACGGAG403300.021.08374834
AAGAACG409550.020.910743
GCGTTCT421200.020.3334149
CAATACG421250.020.3270431
TTCGACG21400.019.758136
ACGGAGA439000.019.41911735
CCCAATA459550.018.76916129
AACGCTA109400.018.73050923
CGGAGAG458750.018.51887736
Produced by FastQC (version 0.11.5)