FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48266723
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG708210.1467284199095099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG227200.015.12386745
GTCACTC337350.013.79448
GGCATAG253850.013.64060535
GGGAGGT364000.013.5259343
AGGTCAC351100.013.4728616
GGTCACT347500.013.3396637
GAGGTCA367300.013.0444525
ATAGGGG266550.013.02243738
ACTCTCC361650.012.94493611
GAGCTTA271150.012.73662344
GGAGGTC375800.012.7324554
TGGCATA286850.012.1812434
ATAGCTC73500.011.93688139
CATAGGG304300.011.55468137
TCACTCT408800.011.5170759
TGCCCGT164500.011.17407644
GCATAGG315950.011.12862736
TGGGAGG336550.011.0785862
CACTCTC427600.010.90568910
ACCCGGC89250.010.4594085
Produced by FastQC (version 0.11.5)