FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56920019
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1014140.17816930103273507No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT729410.12814647865806228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT256250.020.859531
AGACCGC209700.019.7010944
AACGCTA291700.019.68599323
ACGCTAC294400.019.48999824
GAACGAT211800.019.45142745
ACCGCGT213200.019.4409626
TCACGCT303550.019.3888958
CCGGCTT312700.019.267462
GACCGCG218350.018.9824285
CGCTACC304050.018.79077325
CAGACCG221000.018.7751623
CCGCGTT225650.018.3384287
TTCACGC322900.018.3175457
AAAACGC315650.018.28313821
ACGCTCA333450.017.89312410
CGCGTTC232100.017.8384978
GAAAACG335050.017.18423320
AAACGCT338050.017.11847922
CACGCTC347800.017.0514039
AGAACGA250400.016.53432344
Produced by FastQC (version 0.11.5)