FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56920019
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC63400.013.5515026
GTTGTCG55750.013.52330226
TGTCGTT58450.012.7060128
TATAGCT77150.011.485387
GTCGTTC64950.011.29483729
GCTAAGT69800.011.12432414
AGCTTAG177950.010.96418645
ATAGGGG181000.010.93692938
GGCATAG177350.010.93566935
GTCACTC253950.010.9195068
GGTCACT258250.010.78115757
GGGAGGT283000.010.7184883
TCGTTCC71200.010.58690230
GGAGGTC275650.010.4944144
AGGTCAC293850.09.9654886
CGTTCCC75650.09.90405431
GAGGTCA300450.09.8143035
ACTCTCC287300.09.63862911
ACCCGGC77000.09.4349765
AATATAG114550.09.40523055
Produced by FastQC (version 0.11.5)