FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52856476
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4171650.7892410383166673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG131850.012.03490835
AGCTTAG132550.012.00902245
ATAGGGG140950.011.639132538
GTTGTCG24250.011.47825926
GGTCACT216300.010.3792997
GTCACTC219400.010.3245038
CATAGGG154150.010.32244637
GAGCTTA158000.010.23043644
GGGAGGT260350.010.189363
GCATAGG157200.010.07945436
TGGCATA164250.09.82479134
AGGTCAC249300.09.6808536
ACTCTCC241050.09.60043911
GGAGGTC246750.09.5076624
ACCCGGC84700.09.4095195
ACGGACA87300.09.23090518
TCACCCG89200.09.1840093
TCTATAG195250.09.1468943
TAGGGGA249950.08.95155939
GAGGTCA277650.08.8135595
Produced by FastQC (version 0.11.5)