FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40265034
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG15850.018.0278436
GTCCGTT28950.017.6627861
TTCGACG19800.017.6131026
CTCGGTT86000.016.187211
ATGCGCG23550.015.57277544
TCGACGA23400.015.48037
CCCGTAT25950.015.2776751
TGCGCGT23900.015.25081145
AACGCTA94150.015.00756623
ACGCTAC95500.014.86609624
AGACGCT168150.014.31713941
CTCCGAT46900.014.2647961
TCACGCT107600.014.239818
CGCTACC99850.014.12831725
CGACGAG23500.013.8825588
CCCCGCA166850.013.60613635
GTCGGTT36050.013.5592741
TACGGTA63150.013.53878144
GTCCGGT33600.013.4082681
TTCGGCG51900.013.39554744
Produced by FastQC (version 0.11.5)