FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851585

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851585
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40265034
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG498230.12373763300435807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG142100.016.98580745
GGCATAG155900.015.17945235
ATAGGGG164750.014.69156138
GAGCTTA171100.014.08056344
CATAGGG175950.013.60301237
TGGCATA175450.013.56501834
GGGAGGT243250.013.2391613
GCATAGG186500.012.84562136
ACCCGGC71250.012.7923965
GTCACTC237250.012.7112558
GGTCACT233300.012.6949357
ACTCTCC246000.012.27742311
GGAGGTC250950.012.1513844
TAGCGTA8150.011.86843837
ACGGACA78250.011.79080718
TCACCCG84850.011.6966213
CGGACAC79300.011.38091613
TAGGGGA259950.011.3706739
ATAGCTC77600.011.16039639
CACTCTC281100.010.92852710
Produced by FastQC (version 0.11.5)