FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51542456
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT895380.17371698391710322No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG635460.12328865353253636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT172550.025.7241126
GACCGCG176150.025.1042775
AGACCGC177850.025.0160794
CCGCGTT183700.024.1504977
CGCGTTC188800.023.5572688
CAGACCG192350.023.3524653
TTCGACG26700.022.8226596
GAACGAT211600.021.05963545
TCAGACC225750.020.5255662
AATACGG230600.019.1733732
ATACGGA232400.018.95567733
GCGTTCT235050.018.9411169
TACGGAG232450.018.8838134
AGAACGA246200.018.12451444
TCGACGA35100.017.7451977
AACGCTA187850.017.59908923
ACGCTAC188150.017.57148724
CTCAGAC318500.017.2861961
CAATACG262000.017.1244331
CGACGAG35600.016.9903728
Produced by FastQC (version 0.11.5)