FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851588

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851588
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52330033
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT619430.11836988522441788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT182100.018.73078545
AGACCGC181950.018.5836354
TTCGACG18450.017.5577896
CAGACCG194750.017.5357283
ACCGCGT194200.017.5032586
GACCGCG195200.017.3106565
CCGCGTT200950.016.8930077
CGCGTTC204500.016.6218038
TACGGAG215150.015.79883834
CCCGTAT34250.015.7344431
AGAACGA219550.015.60682444
AATACGG219250.015.60600232
GTCCGTT42200.015.6021511
ATACGGA219200.015.58903533
GCGTTCT230550.014.8217569
TCAGACC250350.014.1725462
CAATACG243800.014.10833631
TTCGGCG70900.013.70836444
CTCAGAC306950.013.6560721
CTCCGAT50800.013.6266371
Produced by FastQC (version 0.11.5)