FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52330033
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG570680.10905401110677687No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG177950.015.8781945
GGCATAG201150.013.93268735
ATAGGGG205100.013.88509938
ACCCGGC99600.013.7195995
GAGCTTA212650.013.26389144
GTCACTC292300.012.7244478
CATAGGG225600.012.55699137
TGGCATA231100.012.36867734
GGGAGGT325050.012.3493893
TCACCCG113900.012.333053
GGTCACT298800.012.2062887
GTTGTCG29150.012.18470226
GCATAGG233300.012.15833936
GGAGGTC310950.012.157654
ACGGACA117750.011.79887418
ACTCTCC313100.011.7797811
CGGCCCG124900.011.4796328
AGGTCAC337550.011.231286
CGGACAG123150.011.20867919
ACACGGA120900.011.04489216
Produced by FastQC (version 0.11.5)