FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48465921
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG923580.19056276677379141No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT547580.11298248103033058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC133300.021.3352154
GACCGCG140400.020.208225
ACCGCGT142800.019.9426336
CAGACCG147300.019.475453
TCACGCT289600.018.1520238
CCGCGTT160850.017.774217
GCCGGCT290550.017.0363061
TTCACGC313550.017.0166367
CGCGTTC169100.016.9735228
TTCGACG19650.016.9424486
ACGCTAC301300.016.88293324
AACGCTA301750.016.87801723
CCGGCTT318550.016.824652
ACGCTCA324750.016.46436310
CGCTACC309350.016.40870925
CGTGGTT326750.015.58625941
AAAACGC329450.015.54000921
TCAGACC192300.015.4913962
CGGCTTC368950.015.3861453
CTACACG92300.015.15840845
Produced by FastQC (version 0.11.5)